The results of cultivation of bird schistosomes (Trichobilharzia szidati and Trichobilharzia regenti) show that the cultivation medium routinely used for human schistosomes is also suitable for mass production of early schistosomula of bird schistosomes, including the unique nasal species - T. regenti. The changes observed resemble those present in worms developing in vivo; therefore, the in vitro produced early schistosomula might be used for further studies of host–parasite interactions.