The present paper focus on aspects of tuning a commercial ectomycorrhizal inoculum based on vegetative mycelia (product EctovitTM manufactured by Symbiom, Ltd., Czech Republic) in two cultivation systems for large-scale applications in target conditions: 1) the effect of inoculation during sowing on growth parameters of planting stock of Norway spruce and Scots pine after one season in a forest nursery in Slovakia, and 2) the effect of 1-year pre-inoculation in a nursery on performance of maritime pine seedlings after forest outplanting to the field in a forest site in Portugal.