Abstract
Thanks to the co-operation with Czech and Slovak Transplant Centres and with some of the Departments of Pathology and Forensic Medicine 274 hearts were collected for allograft heart valves (AHV) processing during 1992 -1995. The Cardiac surgeon dissected the aortic valve with the root and the pulmonary artery trunk with the valve.
Tissues were antibiotically (ATB) sterilised in cultivation medium E 199 (24 hours at 37° C). ATB concentrations (mg/ml): Cepharin 0.2, Azlocilin 0.2, Tobramycin 0.08 and Amphotericin B 0.1 for harvesting at post-mortem (P) or Miconazol 0.05 for sterile retrieval during multiorgan harvesting (MOH).
After sterilisation AHV were stored at 4° C. 49 AHV were infected even after ATB treatment -15 of 35 collected at P (43 %) and 35 of 218 procured during MOH (16 %) - (p < 0.01 - ch2 test). After serological screening of the donor and microbiological testing the AHV were released for clinical use.
Most AHV were programmed cooled to the temperature ot liquid nitrogen (- 196° C), in which they were stored at the Allograft Heart Valves Cryobank. Cryoprotection was achieved by 10 % dimethylsulphidoxide.
Conclusion: A technology of harvesting, processing, storage and transportation of AHV, was introduced. It enabled the routine use in many cardiac surgical units.
The AHV Cryobank was established. 131 AHV were used clinically between 1992 and 1995 (transportation as far as 1 300 km). 108 AHV (82.4 %) were used for repair of complex congenital heart defects, while 23 (17.6 %) were used for aortic valve and/or root replacement.