The influence of glucosinolate content variability in the seed and green matter of winter rapeseed on the slug attack to emergenced plant
2004
Abstract
Methodical directions lean against international standard (ISO 1992). Working procedure for content GSL determination in green matter rape is not in principle different from foregoing advancement for rapeseed seed, except preparation way of essence GSL with dirt reduction (Hrncirík K., 1999).
Embankment cca 1,0 g green matter is three times extracted with boiling 70 % solution volume 2 ml methanol. After cleaning closing up reagent is the sample ready for successive enzymatic desulfataction on iontomeniction and afterwards is desulfoglukosinolate content determine with method HPLC-on column with reverse phase with using gradient elution and detection in UV area.