Abstract
Polyethylene (PE) was irradiated with 10 and 63 keV Ar+ ions to fluences of 1 x 10(17) to 3 x 10(19) m(-2) and then it was grafted with aminoacid (alanine). The changes of surface polarity, electrical conductivity, and oxygen concentration were examined on pristine, as-irradiated, and irradiated-grafted PE.
The in vitro adhesion of mice fibroblasts on the modified PE was evaluated 24 hours after inoculation. It was proved that for the PE irradiated at 10 keV ion energy, the presence of chemically bound alanine increases cell adhesion and its homogenity.
For PE irradiated with 63 keV ions, however, the alanine grafting leads to a reduction of the number of adhering cells. It was found that a rising surface polarity increases cell adhesion, but when its value is too high the cell adhesion starts to decrease.
No correlation between electrical conductivity and cell adhesion was observed. In general, higher cell adhesion is observed on modified PE in comparison with pristine one