Abstract
Reumatoid arthritis occurs in 1 % of the population, in women is 2-3 times more frequent than in men. It takes part of autoimunne (autoagressive) diseases and therefore its study is very important.
Rheumatoid arthritis is typically human disease and its modeling is difficult. The most similar animal model of this disease is adjuvant arthritis.
It was induced by the application of heat killed Mycobacterium butyricum, which was injected at the base of the tail of rats Long Evans. The following parameters were evaluated: body weight, volume of hind limbs, in the blood - serum albumin, ratio NO2/ NO3, corticosterone, prolactin, prolactin mRNA, free radicals in the form of TBARS, antioxidative capacity, immunoglobulin.
The intensity of nociception was measured with tail-flick method. Adjuvant arthritis was influenced by several modes: stress provoked by the food restriction, the decreasing of prolactin level with bromocriptin and with boar seminal factor. 40 % food restriction decreases all signs of experimental adjuvant arthritis in rats.
The similar effect has also the boar seminal immunosupressive fraction. The bromocriptin treatment has not similar effect.
We are trying to apply our experimental results in the human rheumatoid arthritis.