Anticonvulsan action of 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo(f)quinoxaline in immature wats: Comparison with the effects on motor performance
Abstract
Anticonvulsant action of 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo(f)quinoxaline (NBQX), a competitive antagonist at non-N-methyl-o-aspartate receptors for excitatory amino acids, was studied in a model of cortical epileptic afterdischarges (ADs) in 12-, 18- and 25-day-old rat pups with implanted electrodes. Electrical stimulation of sensorimotor cortex was repeated four times with 20-min intervals, NBQX (in doses of 10, 30, 60 or 90 mg/kg i.p.) or solvent (dimethyl sulfoxide, 1 ml/kg i.p.) were injected 10 min after the first afterdischarge.
Dimethyl sulfoxide did not change the phenomena recorded; NBQX shortened ADs or at least blocked progressive prolongation observed under control conditions. Intensity of movements accompanying stimulation decreased after NBQX, and clonic movements accompanying ADs were suppressed in a dose-dependent manner.
The highest dose of NBQX disabled the animals; therefore, the action of this drug on motor skills was studied in another group of animals. Even the dose of 30 mg/kg NBQX interfered with motor performance in 12- and 18-day-old rat pups, 25-day-old rat pups were more resistant to this action.
NBQX exhibited only moderate antiepileptic action (suppression of progressive lengthening of ADs) at doses where unwanted side effects were absent.