Abstract
Objective: Measurement of foetal hemoglobin (HbF) has an important clinical value in the diagnosis as well as in characterization of some Hb structural variants and other hemoglobinopathies. We evaluated presumptive HbF quantification in analytical mode for glycated hemogobin (HbA1c) on Tosoh HLC-723 G7 analyser.
Material and Methods: We analysed 63 blood samples and quantified HbF by mode for glycated hemoglobin. The samples were sorted to 3 groups according HbF values: group 1 (n = 24) included HbF values 1-2 % of total hemoglobin (Hb), group 2 (n = 21) included HbF values 2-5 % of total Hb, and group 3 (n = 18) included HbF > 5 % of total Hb.
We simultaneously measured HbF in all samples in mode for β-thalassemia determination on the same analyser. Results: The HbF values between 1-2 % obtained by glycated mode did not correlated with results by β-thalassemia mode (r = 0.241).
The both HbF values between 2-5 % and HbF values > 5 % of total Hb correlated acceptable with results by β-thalassemia mode (r = 0.825 and 0.889 respectively; p < 0.001). Only pathological HbF values higher than 2 % of total Hb have the clinical significance.
These values measured by mode for HbA1c determination is possible to take as correct. On the contrary, all HbF values lower then 2 % of total Hb, measured by mode for HbA1c determination, are not reliable.
Conclusion: We conclude that analytical mode for HbA1c determination is also suitable to use for presumptive combined HbF quantification on Tosoh HLC-723 G7 analyser.