Abstrakt
Both oxidants and antioxidants have been shown to modulate cell proliferation. We studied the effects of hydrogen peroxide and two antioxidants on the rate of proliferation of lens epithelial cells in culture.
Hydrogen peroxide at concentrations higher than 32 µM caused a significant inhibition of proliferation. However, in the concentration range of 0.01-0.5 µM, hydrogen peroxide stimulated the rate of proliferation.
The effect of hydrogen peroxide was dependent on the amount of cells in an individual culture well, indicating decomposition of hydrogen peroxide by cellular enzymes. In order to eliminate the possibility of decomposition of the dose of hydrogen peroxide given as a bolus, we induced continual production of hydrogen peroxide by adding glucose oxidase to the incubation medium.
We found that hydrogen peroxide, generated by 1-50 µU . ml-1 of glucose oxidase significantly increased the rate of cell proliferation. This effect was most apparent at the beginning of the exponential phase of cellular growth.
Glucose oxidase alone (100-500 µU . ml-1) did not produce any effect. The effects of pro-oxidative hydrogen peroxide were compared with the effects of two biologically important antioxidants, α-tocopherol and retinol.
Both antioxidants completely inhibited proliferation at concentrations of 30 µM and higher. In contrast to retinol, the effect of α-tocopherol was dependent on the amount of cells, indicating cellular decomposition of α-tocopherol.
The results document the possibility of redox regulation of cellular proliferation at physiologically relevant reactant concentrations.