Robust carbohydrate dynamics based on sucrose resynthesis in developing Norway spruce somatic embryos at variable sugar supply
Abstract
Growth regulators and carbohydrates are key regulatory factors that affect somatic embryogenesis. Carbohydrates serve as energy and carbon sources, osmotica and osmoprotectants and are important signal molecules. Most information about the role of carbohydrates in somatic embryogenesis in Norway spruce has been obtained with embryos grown on semi-solid media. The aim of the present study was to gain a better understanding of the effects of exogenous carbohydrates through modification of medium components (sugars) and physical state (liquid and semi-solid media). Rafts, floating on liquid medium, were used to allow precise manipulation of carbohydrate availability, though it did not result in the highest embryo yields. Our results indicate the following for Norway spruce somatic embryo development: (1) overall carbohydrate dynamics in somatic embryos cultivated on liquid or semi-solid media were similar; (2) the total carbohydrate content, however, was higher in somatic embryos cultivated on liquid media; (3) sucrose was present in somatic embryos even when they matured on sucrose-free media; (4) sucrose content in liquid sucrose-supplemented maturation media decreased sharply during a 1-wk subculture interval; (5) the accumulation of the raffinose family oligosaccharides during desiccation was determined independently of previous sugar supply; and (6) a decrease of sucrose and an increase of hexoses contents accompanied somatic embryo germination.