Abstrakt
Monitoring the behaviour of biologically active compounds in the body is the key to understand their effect. Optical methods are relatively cheap non-ionizing techniques based on the specific optical properties and are an important tool for non-invasive and objective diagnosis with still better and better resolution.
In this work, a characterization of fluorescence behaviour of doxorubicin in different environments (ethanol, methanol, dimethyl sulphoxide) has been established. In addition, its spatial distribution in muscle tissue was monitored using fluorescence imaging.
Autofluorescence of the tissue was removed by software to limit the effect of the matrix. The novelty of this study lies in the field of sensing of doxorubicin in a tissue.
The results can be base for an optic array, which could be used for sensing if the drug in vivo.