Voltammetric Study of the Interaction of Methyl Violet 2B with DNA and Its Use for the Determination of DNA in Aqueous Solutions
2015
Abstrakt
Differential pulse voltammetry and cyclic voltammetry at a hanging mercury drop electrode (HMDE) were used to study the interaction of methyl violet 2B (MV) with double- stranded DNA in 0.1 mol L-1 acetate buffer pH 4.0 (AB). The mechanism of electrochemical reduction of MV was studied as well, using cyclic voltammetry at HMDE.
Moreover, the calibration dependence of the peak current of MV on the concentration of DNA in AB was constructed to be used for the determination of DNA, based on the fact that the peak current of MV is decreasing with increasing concentration of DNA in solution.