Determination of acid dissociation constants of triazole fungicides by pressure assisted capillary electrophoresis
Abstrakt
Pressure assisted capillary electrophoresis was applied to determination of acid dissociation constants (pK(a)) of six widely used triazole fungicides (cyproconazole, epoxiconazole, flusilazole, tebuconazole, penconazole and propiconazole) in aqueous medium. The pK(a) values were determined from the dependence of effective electrophoretic mobility of the triazole fungicides on p[H+] of the background electrolyte (BGE) using non-linear regression analysis.
The p[H+] was used instead of pH to reflect the increased ionic strength of the strongly acidic BGEs (pH< 1.75) as compared to the BGEs at pH equal to or greater than 1.75. Prior to the pK(a) calculation, the measured effective electrophoretic mobilities were corrected to the reference temperature (25 degrees C) and constant ionic strength (25mM).
The regression function was modified to allow the determination of pK(a) in the BGEs of varying ionic strength. The electrophoretic measurements showed that the above triazole fungicides are very weak bases their pK(a) values were found to be in the range 1.05-1.97 and were in a good agreement with the values calculated by SPARC online pK(a) calculator. (C) 2015 Elsevier B.V.
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