Abstrakt
A comparison of SFC-MS and LC-MS in a field of bioanalysis. The advantages of supercritical fluid chromatography (SFC) over liquid chromatography (LC) have been widely described in the past and include i) enhanced kinetic performance, due to the lower fluid viscosity and better solute diffusion coefficient, ii) lower consumption of organic solvents, iii) highly efficient chiral separation in presence of a supercritical fluid, and iv) improvement of productivity at the preparative scale.
SFC-MS appears as a viable alternative to LC-MS for bioanalytical applications. Indeed, it can be considered as a faster, greener, more universal and orthogonal analytical method, compared to LC-MS.
However, the number of studies showing a systematic comparison of sensitivity, matrix effects, linearity, precision and accuracy with different biological fluids in SFC-MS and LC-MS is still too limited to draw reliable conclusions. The preliminary data obtained on urine and discussed in this editorial prove that SFC-MS is often superior to LC-MS in these aspects.
In the future, there is a need to extend this work to prove its applicability and to transform SFC-MS into a mature technique for bioanalysis.