Publication at Faculty of Science, Central Library of Charles University |
2015
Abstract
In this contribution, the construction and preparation of 3 types of flow amperometric enzymatic biosensors based on silver solid amalgam (AgSA) were presented. Two of them consist of the tubular detector and enzymatic reactor of porous or powdered AgSA.
The third biosensor was constructed using polished AgSA electrode. Reactors and polished AgSA electrodes were modified with thiols or chitosan and different enzymes were covalently immobilized by suitable techniques.
Prepared biosensors were used for determination of ascorbic acid, glucose, hydrogen peroxide, catechol, pyrogallol, dopamine and sarcosine in model samples and in same cases in the real samples.