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The Technology of Digital PCR for Analysis of The Deletion Polymorphism in the GSTM1 Gene

Publication at Faculty of Medicine in Hradec Králové, Faculty of Pharmacy in Hradec Králové |
2016

Abstract

The aim of this study was to perform genetic analysis of deletion polymorphism in the GSTM1 gene by using the technology of digital PCR. For genotyping, the QX100 Droplet Digital PCR System was used.

The absolute quantity of GSTM1 copies was normalized to the beta-globin reference gene. Digital PCR seems to be an available and reliable technology for GSTM1 deletion polymorphism genotyping.