Histological evaluation of the healing of abdominal incisions in the pig - an in vivo experiment using tissue scaffolds
Abstract
Several histological methods are currently used to assess the incorporation of artificial or biological grafts in animal models of incisional hernia repair. Histological incorporation of the grafts is supposed to correspond with mechanical quality of the mesh-tissue interface, as tested in a porcine model by Majercik et al. (2006) and Jenkins et al. (2010).
At the Department of Histology and Embryology, Faculty of Medicine was delivered 14 samples of pig abdominal walls fixed in formalin (Prolene - 6 and composite - 8). It was found that in the site of incision lower inflammatory encapsulating of the stitches and the remaining material mesh in samples with composite mesh than in samples with Prolene mesh.
Also, collagen type I was evenly distributed in the healing incision and was less connective tissue than fat permeated at Composite samples compared with Prolene samples, although this difference was not significant quantitatively.