The pyruvate, orthophosphate dikinase (PPDK, EC 2.7.9.1), crucial plant enzyme in the process of concentrating CO2 for Calvin cycle, catalyzes phosphoenolpyruvate-regeneration phase of the C4 photosynthetic pathway [1]. However plants possess also non- photosynthetic isoform of PPDK, which functions are less understood, probably due to its low abundance [2].
In all plants, PPDK is located in both cytoplasmic and plastidic compartments [3]. PPDK catalyzes the reversible ATP- and Pi- dependent formation of phosphoenolpyruvate from pyruvate [4,5].