Abstract
In remediations, bioremediation is used in final phase for residual decontamination or it can be also used as an independent remediation technique. Therefore it is worth to know how the remediation techniques influence toxic effect on organisms.
Usual ex situ and in situ methods for examining toxicity of the underground environment bring certain disadvantages, especially conditions differing from natural conditions, use of model organisms which are naturally not occuring in a given environment or use of materials which can influence the growth of microorganisms. The aim of this work was to design a device for monitoring of toxic effect of remediation techniques which would exclude disadvantages of known techniques and would be suitable for rapid, effective and cheap in situ verification of toxicity.
We developed a device made up from a perforated vessel consisting of an inert material, the filling for microbial growth is formed by soil from the rock environment and represents natural conditions. Biomass is formed by autochthonous microorganisms naturally occuring in a given rock environment.
The device consists of five perforated vessels which can be removed in different time intervals and thus it is possible to observe how the microorganism composition changes in time.