Spectrometric methods for determination of ligand-protein ratio in monoclonal conjugates: a mini review

Abstract

An overview is provided of spectrometric methods for determination of the number of ligand molecules per protein molecule in monoclonal conjugates used in radiodiagnostics or radiotherapy. Individual methods (UV/Vis, fluorescence, and infrared spectrometry) are discussed and examples of their application described.

Keywords

• Analytical methods
• Monoclonal conjugates
• Ligand-antibody ratio