The complete mitochondrial genome of the Ukrainian nine-spined stickleback Pungitius platygaster was obtained using massive parallel sequencing of genomic DNA. The mitogenome sequence was 16 570 bp long, and the gene order and contents were identical to those of other sequenced Pungitius mitogenomes.
In a phylogenetic analysis, the mitogenome of P. platygaster clustered with other Pungitius mitogenomes, yet being clearly distinct from those of P. pungitius, P. sinensis and P. kaibarae.