Abstract
"Colostrum is a rich source of essential nutrients for newborn infants. Therefore, our aim was to assess the possibility of combining bovine colostrum with probiotics and prebiotics.
First, growth of selected probiotic strains from genera Bifidobacterium, Lactobacillus and Enterococcus in bovine colostrum were tested. Bacterial growth in colostrum was compared on the basis of cell counts to determine by agar plate technique after 24-hr cultivation.
In other step, we investigated the immunomodulatory effect of bovine defatted or fatted colostrum by the 3-day in vitro stimulation of human peripheral blood mononuclear cells (hPBMCs). Luminex multiplex analysis was used to analyze cytokine production by the hPBMCs.
A statistically significant difference of the effect of various bioactive substances on microbial growth has not been confirmed. Enterococcus faecium CCDM 922 grew best from all selected strains in bovine colostrum (P < 0,05).
Our results has shown that bovine defatted colostrum stimulated the secretion of Th2 cytokines IL-10, IL4 and IL-5 which inhibited the production of Th1 cytokines. "