Determination of 5-hydroxyindole-3-acetic acid using Flow Injection Analysis with Electrochemical Detection
2017
Abstrakt
Tumour biomarker, 5-hydroxyindole-3-acetic acid, has been determined at screen-printed carbon electrodes using flow injection analysis with amperometric detection. Britton-Robinson buffer (0.04 mol.l-1, pH 2.0) was used as an optimum carrier solution.
Dependence of the peak current on the analyte concentration was linear in the whole tested concentration range (from 0.05 to 100 μmol.l-1) with limits of detection 0.033 μmol.l-1 (calculated from peak heights) and 0.012 μmol.l-1 (calculated from peak areas).