Abstract
The aim of the project was the development of a technological process for the production of a new nanofibrous-based coating on invasive blood vessel inputs, which would allow for a prolongation of the interval between catheter exchange in patients with established CVC and PVC, to prolong the period between two change dressings, thus contributing to the reduction of nosocomial infections. A special cap on the basis of nanofibres activated by the photosensitizer allows the formation of singlet oxygen, which has strong disinfecting effects and does not give rise to resistance.
Singlet oxygen production was demonstrated in vitro with a nanofibrous membrane at the most suitable thickness of 7μm. Its production has proven bactericidal effects after light exposure, respectively 10 minutes for commonly found bacteria on the human skin.
In vivo tests (on the model of human skin and on laboratory mouses) have shown that the nanopowder membrane adduced by TPP is not irritating for the skin, non-sensitizing, non-phototoxic, and non-penetrating through the skin. Thus, it has the prerequisites for use in clinical practice as a medical device.