In sequencing technologies of nucleic acids, significant progress has been made in recent years, and the use of these methods is slowly but surely endorsed in the practice of clinical oncologists. Next-generation sequencing (NGS) is relatively fast and reliable method and nowadays it became to be also financially available.1 The main idea of personalized oncology is prediction of response to targeted treatment based on molecular markers independently to histological finding.
And for this approach the understanding of molecular methods is necessary.2 Therefore, we decided to show possibilities of NGS and its implication to daily practice in this article.