Abstract
Elevated concentrations of exogenous biotin in serum makes interferences in immunoanalytical measurements based on using strepravidin-biotin linkage. Interferences are negative in cases of noncompetitive methods and positive in competitive methods.
Elevation of serum biotin causes its use in therapy and very often in cases using as food supplements. Elevation of biotin may be 300-10000 times higher than concentration without addition of exogenous biotin.
Interferences of biotin are extremely dependent on method and analytes. Potential interferences are possible in more than 50 analytes measured by immunoanalytical methods, namely of steroid hormones, cardiac markers, tumour markers, some therapeutic drugs and others.
Necessity of biotin interference problem lead to creating some important guidance's, warnings and requirements introduced in this work.