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Comparison of Metallothionein Determination by Brdicka Reaction and ELISA

Publikace na 2. lékařská fakulta |
2010

Tento text není v aktuálním jazyce dostupný. Zobrazuje se verze "en".Abstrakt

In this study, two methods for metallothioneins (MT) determination in a biological sample were compared. 25 human, 9 pig blood serum samples and liver and kidney samples from 35 carp (Cyprinus carpio) were analyzed by ELISA and differential pulse voltammetry Brdicka reaction. The results obtained by these two methods were in good agreement.

For commercially available MT standard the correlation coefficients between the single concentrations signal height was 0.99. In biological samples the correlation coefficients were 0.90 for fish liver and kidney samples, 0.91 for pig blood serum and 0.93 for human blood serum.