Abstract
The study is focused on optimization and validation of methods for determination of toxic indoxyl sulfate in human urine and blood serum. Samples were analyzed using HPLC/ED-DAD system after the biological matrix had been pretreated.
Validated methods were successfully tested on samples from healthy people as well as from dialyzed patients. The aim was to monitor both indoxyl sulfate levels and impact of various methods for elimination of this toxin from patients with renal disease.