In vitro antifungal susceptibility patterns of Trichophyton benhamiae complex isolates from diverse origin
Abstrakt
Background Species from the Trichophyton benhamiae complex are mostly zoophilic dermatophytes which cause inflammatory dermatophytosis in animals and humans worldwide. Objectives This study was purposed to (a) to identify 169 reference and clinical dermatophyte strains from the T benhamiae complex species by molecular method and adhering to the newest taxonomy in the complex (b) to evaluate the in vitro antifungal susceptibility profile of these strains against eight common and new antifungal agents that may be used for the treatment of dermatophytosis.
Methods All isolates, mainly originated from Europe but also from Iran, Japan and USA, were subjected to ITS-rDNA sequencing. The in vitro antifungal susceptibility profiles of eight common and new antifungal drugs against the isolates were determined by CLSI M38-A2 protocol and according to microdilution method.
Results Based on the ITS-rDNA sequencing, T benhamiae was the dominant species (n = 102), followed by T europaeum (n = 29), T erinacei (n = 23), T japonicum (n = 10), Trichophyton sp (n = 4) and T eriotrephon (n = 1). MIC ranges across all isolates were as follows: luliconazole: 0.0002-0.002 mu g/ml, terbinafine: 0.008-0.125 mu g/ml, efinaconazole: 0.008-0.125 mu g/ml, ciclopirox olamine: 0.03-0.5 mu g/ml, itraconazole: 0.06-2 mu g/ml, griseofulvin: 0.25-4 mu g/ml, amorolfine hydrochloride: 0.125-4 mu g/ml and tavaborole: 1-16 mu g/ml.
Conclusion Luliconazole, efinaconazole and terbinafine were the most potent antifungals against T benhamiae complex isolates, regardless of the geographic locations where strains were isolated. These data might help dermatologists to develop effective therapies for successful treatment of infections due to T benhamiae complex species.