Abstract
Capillary electrophoretic separation of aesthetic ketamine and its metabolites norketamine, hydroxynorketamine, and dehydronorketamine is performed under influence of opposite electroosmotic flow. Electrophoretic separation is realized in covalently coated fused silica capillaries covered by copolymer of neutral acrylamide (PAM)/cationic 3-acrylamidopropyl trimethylammonium chloride (APTAC).
The content of APTAC in polymerization mixture changed in range 0 - 6 % and the generated electroosmotic flow changes in interval 0 - 20 x 10-9 m2/V.s. The determination of ketamine and its derivates in rat serum is performed in 4% PAMAPTAC capillary with inner diameter of 25 µm in 500 mM aqueous solution of acetic acid (pH 2.3) as background electrolyte.
LOD ranges from 2.2 ng/mL for dehydronorketamine to 4.1 ng/mL for hydroxynorketamine. New method is applied for the time monitoring of ketamines in rat serum after the intra venous administration of low dose of anaesthetic at level 2 mg/kg.