Abstract
Plasma membranes as well as their simplified model systems show an inherent nanoscale heterogeneity. As a result of strong interleaflet interactions, these nanoheterogeneities (called here lipid nanodomains) can be found in perfect registration (i.e. nanodomains in the inner leaflet are registered with the nanodomains in the outer leaflet).
Alternatively, they might be inter-leaflet independent, anti-registered or located asymmetrically in one bilayer leaflet only. To distinguish these scenarios from each other appears to be an experimental challenge.
In this work, we analysed the potential of Förster resonance energy transfer (FRET) to characterize inter-leaflet organization of nanodomains. We generated in-silico time-resolved fluorescence decays for a large set of virtual as well as real donor/acceptor pairs distributed over the bilayer containing registered, independent, anti-registered or asymmetrically distributed nanodomains.
We focused on membrane characteristics as well as the characteristics of fluorescent probe to determine those with the highest impact on resolution. In this way, we were able to identify conditions that gave satisfactory or unsatisfactory resolution.