Non-Thermal Plasma Treatment Improves Properties of Dormant Seeds of Black Locust (Robinia pseudoacacia L.)
Abstract
This article deals with the breaking down of seed dormancy in black locust (Robinia pseudoacacia L.) seeds. These seeds have a hard testa that is impermeable to water and air.
In this investigation, seeds of black locust were treated with non-thermal plasma (NTP) for 0, 5, 10, and 20 min. The testa of the treated seeds had an eroded surface (SEM analysis) and showed an increased oxygen content (EDS) and increased hydrophilicity (contact-angle measurement).
The exposed seeds also had a higher water absorption (seed water uptake) than the untreated ones. In seeds treated for 20 min, a significant difference (p < 0.05) was recorded in seed germination (eight times greater than the control set) and in other parameters of initial seedling development, such as the fresh weight of both seedling root and shoot and all three indexes of seedling vitality.
The detected changes in the seed surface and changes in germination proved that NTP can partly break dormancy in black-locust seeds.