Abstract
The main goals of this study were to demonstrate that inhibition of NF-kB by sodium salicylate decreases the viability of rat phaeochromocytoma PC12 cells and to investigate the nature of cell damage and death. PC12 cells were treated with different concentrations of sodium salicylate (1-20 mM).
Higher concentrations (10-20 mM) killed PC12 cells in a dose-dependent manner. The assessments were done by direct cell counting in a Bürker chamber and by the WST-1 cytotoxicity assay.
We also found a decreased NF-kB activity after sodium salicylate treatment by electrophoretic mobility shift assay (EMSA). The cells treated with sodium salicylate were undergoing apoptosis as seen on our records obtained by time-lapse videomicroscopy as well as shown by DNA fragmentation experiments.
The decreased DNA binding activity of NF-kB indicates that the inhibition of NF-kB can play a role in these processes.