Comparison of various methods used for extraction of cell-free genomic DNA from human plasma

Abstract

For many years it has been known that free DNA of various origin is released into peripheral blood. An extremely short halftime of plasma DNA and many pre-analytical variables may affect the number of free DNA molecules in plasma specimens.

The goal of our study was to compare six nucleic acids extraction protocols to define the optimal pre-analytical approach for further molecular analyses based on plasma DNA

Keywords

• DNA
• genomic DNA
• free-cell DNA
• extraction
• nucleic acids
• plasma
• PCR
• pre-analytical phase
• spectrophotometry
• electrophoresis