Effective Removal of Nonionic Detergents in Protein Mass Spectrometry, Hydrogen/ Deuterium Exchange, and Proteomics

Abstract

Detergents are frequently used for protein isolation and solubilization. Their presence is crucial in membrane protein protocols or in lipid raft proteomics.

However, they are usually poorly compatible with mass spectrometry. Several different sample preparation protocols are routinely used, but they are either laborious or suffer from sample losses.

Keywords

• Integral membrane-proteins
• enzymatic digestion
• surfactants
• peptides
• extraction
• complexes
• bacteriorhodopsin
• crystallization
• optimization
• enrichment