Introduction to Medical Microbiology, Basic Disciplines. Role in Health Care System. Characteristics of microorganisms- bacteria, viruses, fungi and parasites

Identification Techniques for Microbes

Morphology and Physiology of Bacteria, Disinfection and Sterilisation Technics, Methods of Sterility Control

Interaction of bacteria and the host - Pathogenicity and Virulence,

Antimicrobial agents and antimicrobial therapy.

Review of Medical Bacteriology.Bacterial Genetics

Gram-negative Bacteria, Spirochets and Mycoplasma

Mycobacteria, Chlamydiae, Rickettsiae

Viruses. Taxonomy. pathogenesis and intracellular parasitism in the host cell

Selected DNA Viruses

Prions and prionic infections

Selected RNA Viruses

Medical Mycology

Parasitology

Seminars (Attendance Compulsory):

Diagnostic Techniques in Medical Microbiology
Rational antimicrobial therapy

Practicals:

Microscopy in medical bacteriology, mycology and parasitology. Native, fixed and stained slide. Staining techniques (Gram. Ziehl-Nielsen, Buri, Giemsa, immunofluorescence). Bacterial culture media, cultures and macroscopic morphology. Metabolic Tests (API, Pliva Staphytest, Streptotest, Enterotest) Toxin detection. Antigen detection, Typing. Antibiotic susceptibility tests (diffusion/disc tests, E-tests, MIC, MBC-testing) Serologic reactions( agglutination, precipitation, immunodiffusion, immunofluorescence, ELISA, immunoblot). Genome detection techniques ? hybridisation, PCR. Horizontál electrophoresis, real-time PCR.
Urine culture and interpreting of the test. Yeast culture (Sabouraud) and GT-test.
Sample collection techniques and Kits, documentation, transport requirements.
Disinfection and Sterilisation techniques and Sterility control.
Blood cultures ? proper taking of samples, culture systéme icl. BacTec, BactAlert.
Anearobic Culture. Anaerostat.